• For academic and research usage, please contact the Ambystoma genomic stock center. 
• In the interest of the salamander research community, we may supply transgenic lines upon request. Please contact “pmurawala@mdibl.org“, if you are interested in any of the below listed transgenic lines.
• We do not supply axolotls to private individuals. 

Disclaimer!!! 

• Many of these lines are developed by researchers that do not belong to our lab.
• Please cite and credit the transgenic lines appropriately as each line takes effort to generate stable F1/F2 lines and to thoroughly characterize them. 
• Please also visit www.axobase.org for all published lines in the field.

The following transgenic animals are available in our laboratory at MDIBL.

Regular strains

1)         d/d strain – A white animal with black eyes (Order from AGSC)

2)         Wild type animals – Black animals (Order from AGSC)

Ubiquitous fluorescence for grafting studies

3)         tgSceI(CAGGs:eGFP)Etnka – Ubiquitous GFP expression (Sobkow et al, Developmental Biology, 2006) (Order from AGSC)

4)         tgSceI(CAGGs:CherryNuc)Etnka – Ubiquitous nuclear Cherry expression (Kragl et al, Nature, 2009) 

5)         tgSceI(CAGGs:lifeAct)Pmx – To visualize actin cytoskeleton (Unpublished)

6)         tgSceI(CAGGs:Alpha-Tubulin)Pmx – To visualize microtubules (Unpublished)

Fluorescent reporters

7)         tgSceI(Mmu.B3Tub:memGFP)Etnka -> For visualizing neurons (Khattak et al, Stem Cell Reports, 2013) (Order from AGSC)

8)         tgSceI(Mmu.B3Tub:Cherry)Pmx -> For visualizing neurons (Unpublished)

9)         tgSceI(Xla.Caract:GFP)Etnka -> For visualizing muscle fibers (Khattak et al, Stem Cell Reports, 2013)  (Order from AGSC)

10)      tgSceI(Mmu.CNP:GFP)Etnka -> For visualizing Schwann cells (Khattak et al, Stem Cell Reports, 2013) (Order from AGSC)

11)      tgSceI(RARE:GFP)Pmx -> For visualizing retinoic acid activity (Monaghan et al, Developmental Biology, 2012)

12)      tm(Sox2t/+:Sox2-T2A-Cherry)Etnka -> For visualizing neural progenitors (Fei et al, PNAS, 2017)

13)      tm(HoxA13t/+:HoxA13-T2A-Cherry)Etnka -> For visualizing hand plate cells (Oliveira et al, Nature Communications, 2022)

14)      tgSceI(TRE:GFP)Pmx -> For visualizing thyroid pathway activity (Unpublished)

Cre drivers

15)      tgSceI(CAGGs:ERT2-Cre-ERT2-T2a-GFPnls)Etnka -> Ubiquitous inducible Cre driver (Khattak et al, Stem Cell Reports, 2013) (Order from AGSC)

16)      tgSceI(Mmu.Col1A2:TFPnls-T2A-ERT2-Cre-ERT2)Etnka -> For labeling various subtypes of connective tissue cells across the body (Gerber et al, Science,  2018) (Order from AGSC)

17)      tm(Pax7t/+:Pax7-P2A-memCherry-T2A-ERT2-Cre-ERT2)Etnka -> For labeling satellite cells and Pax7 domain of the neural tube (Fei et al, PNAS, 2017) (Order from AGSC)

18)      tgSceI(Mmu.Prrx1:TFPnls-T2a-Cre-ERT2)Etnka -> For labeling limb mesenchyme derivatives (Gerber et al, Science,  2018) (Order from AGSC)

19)      tm(Sox2t/+:Sox2-P2A-memCherry-T2A-ERT2-Cre-ERT2)Etnka -> For labeling neural progenitors and lateral line cells (Fei et al, PNAS, 2017)

20)      tm(Twist3t/+:Twist3-T2A-GFPnls-T2A-ERT2-Cre-ERT2)Pmx -> For labeling dermal fibroblasts (Masselink et al, BioRxiv, 2024)

21)      tgSceI(Mmu.Lfng:GFPnls-T2a-Cre-ERT2)Pmx -> For labeling asomitic stem cells (Masselink et al, BioRxiv, 2024)

22)      tm(Sox9t/+:Sox9-T2A-GFPnls-T2A-ERT2-Cre-ERT2)Pmx -> For labeling skeletal cells (Unpublished)

23)       tm(MCKt/+:MCK-T2A-GFPnls-T2A-ERT2-Cre-ERT2)Pmx  -> For labeling skeletal muscles and cardiac muscle (Unpublished)

24)      Inducible Cre line -> For labeling hand plate fibroblasts (Unpublished)

25)      Inducible Cre line -> For labeling neural progenitor cells (Unpublished)

26)     Inducible Cre line -> For labeling neurons (Unpublished)

LoxP reporters

27)      tgSceI(CAGGS:Brainbow 2.1)Etnka-> Brainbow reporter (Currie et al, Dev Cell, 2016)

28)      tgSceI(CAGGs:LoxP-GFP-LoxP-Cherry)Etnka -> GFP to Cherry reporter (Khattak et al, Stem Cell Reports, 2013) (Order from AGSC)

29)      tgSceI(CAGGs:LoxP-GFP-dead (STOP)-LoxP-Cherry)Etnka -> No label to Cherry reporter (Kawaguchi et al, Dev Cell, 2024)

30)      tgSceI(CAGGs:LoxP-Cherry-LoxP-GFP)Etnka -> Cherry to GFP reporter (Unpublished)

31)      tgSceI(CAGGs:LoxP-BFPnls-LoxP-Cherry-T2A-NTR2.0)Pmx -> Conditional cell ablation line that uses metronidazole (Unpublished)

32)      tgSceI(CAGGs:LoxP-GFP-LoxP-DTR)Pmx -> Conditional cell ablation line that uses diphtheria toxin (Unpublished)

33)      tgSceI(CAGGs:LoxP-BFPnls-LoxP-mEOS2)Pmx -> Conditional photoconvertible reporter (Unpublished)

34)      tm(Tyr733v6D4/733v6D4)Etnka -> Tyrosinase gene (Fei et al, Stem Cell reports, 2014)